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  2. Online measurement data of continuous aqueous two-phase flotation (ATPF) for enzyme purification
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    Dataset: Online measurement data of continuous aqueous two-phase flotation (ATPF) for enzyme purification

    • RADAR Metadata
    • Content
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    • Technical Metadata
    Alternate identifier:
    -
    Related identifier:
    (Is Identical To) https://publikationen.bibliothek.kit.edu/1000174648 - URL
    Creator/Author:
    Lohfink, Kim Carina https://orcid.org/0009-0009-1321-7720 [Institut für Mechanische Verfahrenstechnik und Mechanik (MVM), Karlsruher Institut für Technologie (KIT)]

    Kirsch, Michel [Institut für Mechanische Verfahrenstechnik und Mechanik (MVM), Karlsruher Institut für Technologie (KIT)]

    Nirschl, Hermann [Institut für Mechanische Verfahrenstechnik und Mechanik (MVM), Karlsruher Institut für Technologie (KIT)]
    Contributors:
    -
    Title:
    Online measurement data of continuous aqueous two-phase flotation (ATPF) for enzyme purification
    Additional titles:
    -
    Description:
    (Technical Remarks) - The .csv files contain experimental data obtained from ATPF experiments at laboratory scale. - The flotation tank used contains 700 ml of the aqueous two-phase system (ATPS). The phase forming components were polyethylene glycol 1000 and trisodium citrate dihydrate. - Phospholipase A2 was used as ... - The .csv files contain experimental data obtained from ATPF experiments at laboratory scale. - The flotation tank used contains 700 ml of the aqueous two-phase system (ATPS). The phase forming components were polyethylene glycol 1000 and trisodium citrate dihydrate. - Phospholipase A2 was used as the model enzyme and the model suspension was mixed with the bottom phase. - The flotation tank was conceptually divided into three compartments, each compartment containing a gassing unit and an electrical conductivity probe. The electrical conductivity probes were inserted 1 cm below the phase interface. - An UV/Vis spectrometer with flow cell was implemented at the top phase outlet to obtain the top phase concentration in real-time. The bottom phase concentration was calculated via a mass balance equation. - The file names contain the process parameters in the following order: feed concentration_gas flow rate combination (Vd_1-Vd_2-Vd_3, see also below)_residence time_volume ratio (top to bottom phase)_experiment number. - The .csv files contain the following experimental data: - t: time in min - R_top, R_bot: top and bottom phase recirculation (R = 1 for full recirculation, R = 0 for no recirculation) - c_bot_3, c_top_3: enzyme concentration obtained from UV/Vis measurements in % (w/w). - kappa_bot_1, kappa_bot_2, kappa_bot_3: Electrical conductivity measurements in all three compartments in mS/cm. - Qd_bot, Qd_top: Volume flows of top and bottom phase in ml/min. - Vd_1, Vd_2, Vd_3: Gas volume flows of the three gassing units in ml/min.
    • The .csv files contain experimental data obtained from ATPF experiments at laboratory scale.
    • The flotation tank used contains 700 ml of the aqueous two-phase system (ATPS). The phase forming components were polyethylene glycol 1000 and trisodium citrate dihydrate.
    • Phospholipase A2 was used as the model enzyme and the model suspension was mixed with the bottom phase.
    • The flotation tank was conceptually divided into three compartments, each compartment containing a gassing unit and an electrical conductivity probe. The electrical conductivity probes were inserted 1 cm below the phase interface.
    • An UV/Vis spectrometer with flow cell was implemented at the top phase outlet to obtain the top phase concentration in real-time. The bottom phase concentration was calculated via a mass balance equation.
    • The file names contain the process parameters in the following order: feed concentration_gas flow rate combination (Vd_1-Vd_2-Vd_3, see also below)_residence time_volume ratio (top to bottom phase)_experiment number.
    • The .csv files contain the following experimental data:
      • t: time in min
      • R_top, R_bot: top and bottom phase recirculation (R = 1 for full recirculation, R = 0 for no recirculation)
      • c_bot_3, c_top_3: enzyme concentration obtained from UV/Vis measurements in % (w/w).
      • kappa_bot_1, kappa_bot_2, kappa_bot_3: Electrical conductivity measurements in all three compartments in mS/cm.
      • Qd_bot, Qd_top: Volume flows of top and bottom phase in ml/min.
      • Vd_1, Vd_2, Vd_3: Gas volume flows of the three gassing units in ml/min.
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    Keywords:
    -
    Related information:
    -
    Language:
    -
    Publishers:
    Karlsruhe Institute of Technology
    Production year:
    2024
    Subject areas:
    Engineering
    Resource type:
    Dataset
    Data source:
    -
    Software used:
    -
    Data processing:
    -
    Publication year:
    2024
    Rights holders:
    Lohfink, Kim Carina https://orcid.org/0009-0009-1321-7720

    Kirsch, Michel

    Nirschl, Hermann
    Funding:
    -
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    Name Storage Metadata Upload Action
    Status:
    Published
    Uploaded by:
    kitopen
    Created on:
    2024-10-01
    Archiving date:
    2024-10-08
    Archive size:
    170.5 kB
    Archive creator:
    kitopen
    Archive checksum:
    3d520805a7c220ee3280d3e77e0546c3 (MD5)
    Embargo period:
    -
    DOI: 10.35097/pn5npep7vfjn92fe
    Publication date: 2024-10-08
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    This work is licensed under
    CC BY 4.0
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    Cite Dataset
    Lohfink, Kim Carina; Kirsch, Michel; Nirschl, Hermann (2024): Online measurement data of continuous aqueous two-phase flotation (ATPF) for enzyme purification. Karlsruhe Institute of Technology. DOI: 10.35097/pn5npep7vfjn92fe
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